| Pak. J. Bot., 18(1): 21-27. | Back to Contents | ||||
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Updated: 10-12-12 | ||||
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STUDIES ON IN VITRO CULTURE OF ARACHIS HYPOGAEA HYPOCOTYL EXPLANTS IHSAN ILAHI AND SHAUKAT AMEEN Abstract: Arachis hypogaea hypocotyl explants exhibited callus formation when inoculated on Murashige & Skoog (MS) medium supplemented with both an auxin and cytokinin. Although best callus resulted in 0.5 mg/1 of 2, 4-D and 1.0 mg/1 of K but it did not support further callus proliferation. 2, 4-D @1.0 mg/1 and K @ 4.0 mg/1 supported callus growth. Browning of callus tissues in subcultures for long duration was overcome by the addition of coconut milk (CM) and sodium diethyl dithio carbo¬nate (SDDC). Department of Botany, University of Peshawar, Peshawar, Pakistan. |
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