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ESTABLISHMENT OF REGENERATING CALLI AND CELL-SUSPENSION LINE OF BASMATI
RICE (ORYZA-SATIVA L CV B.370)
ZAFAR Y; WAJID A; MALIK KA; et al.
Abstract:
Methods were developed for callus induction and efficient plant
regeneration from mature embryos and immature inflorescences of basmati
rice (Oryza sativa L cv. Basmati-370). Callusing from seeds of rice was
observed in MS or N6 media with various levels of 2,4-D. Somatic
embryogenesis was achieved in both media however, response was better in
the presence of Kinetin (2 mg l-1). Using field grown immature
inflorescences and embryos induction of callus and regeneration was
observed. Cell suspension cultures were initiated from 2-3 months old
calli of B-370. A fast growing and finely dispersed cell line of
Basmati-370 was established. Regeneration of plants was achieved in MS-0
(no hormone).
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