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PLANT-REGENERATION IN MESOCOTYL CALLUS OF BRASSICA-JUNCEA VAR POORBIRAYA
SHAHZADI N; AHMAD Z; HUSSAIN A; et al.
Abstract:
Mesocotyl segments of Brassica juncea var. poorbiraya from in vitro
grown seedlings were tested for their callogenic and thence morphogenic
potential with various treatments of plant growth regulators. Calli
grown at 1.0 mg/l of BAP gave a maximum frequencey(2.25 buds/culture) of
shoot regeneration. Regenerated buds were induced to root when cultured
on half strength MS medium containing IAA @ 0.5 mg/l. Embryogenesis was
induced in mesocotyl callus in MS medium containing 2,4-D (0.022 mg/l) +
KIN (0.215 mg/l). Phenotype of regenerated plants looked similar to in
vitro grown Brassica juncea plants.
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