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  Pak. J. Bot., 25(2): 193-198, 1993.

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  Updated: 09-12-10
   

CALLUS CULTURES OF ROSA HYBRIDA CVS., DIAMOND JUBLY AND LANS FRANCE

SHAHZAD HAMEED, ZAHEER AHMAD *, F. Z. KHAN AND MAHMOOD AKRAM*

Abstract: Callus cultures were initiated from flower cups of cultivars of Rosa hybrida. Copious callus formation and growth was observed in cv. Diamond Jubly with 0.5 mg/l 2,4-D + 0.1 mg/1 kn and in cv. Lans France with 0.5 mg/1 2,4-D + 0.5 mg/I kn. Sodium diethyldithiocarbamate (SDC) at 250 mg/1 effectively controlled browning in cultures of both cultivars. SDC effect was related to concentration of phytohormones required by cultures at every 24 day cycle.


Faculty of Pharmacy, University of the Punjab, Lahore, Pakistan.

Pakistan Council of Scientific & Industrial Research, Lahore-54600, Pakistan.


   
         
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