| Pak. J. Bot., 26(1): 191-195, 1994. | Back to Contents | ||||
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Updated: 25-11-10 | ||||
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PLANT REGENERATION FROM CARICA PAPAYA CV. MALIR GROWN IN TISSUE CULTURE K. KHATOON AND R. SULTANA Abstract: Petiole segments and excised epidermal thin cell layers of Carica papaya N. Malir were cultured on full strength MS medium containing a range of concentrations of nephthalene acetic acid (NAA) and isopentenyl adenine (2iP) used seperately and in combination. Petiole segments and epidermal thin cell layers formed maximum callus on medium containing 2.5 mg/1 NAA plus 0.5 mgll2iP and 1 mg/1 NAA plus 1 and 5 mgll 2iP respectively. Enhanced callus growth and shoot formation occurred on half strength macro inorganic salts of MS medium containing 2.5 mg/1 NAA plus 0.5/1 mgll 2iP alongwith 15% coconut milk. Regenerated shoots produced roots on half strength macroinorgani<; salts containing MS medium supplemented with 5 g/1 glucose. The method may be useful for the mass propagation of female papaya plants for large scale cultivation. Department of Botany, University of Karachi, Karachi-32, Pakistan. |
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