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IDENTIFICATION OF DIFFERENT WHEAT GENOTYPES THROUGH POLYMORPHISM BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

S. FAROOQ, T.M. SHAH, E. ASKARI, A.A. ZAIDI, M. ARIF AND N. IQBAL

Abstract: Polymerase chain reaction was used to detect polymorphism in 10 different salt-tolerant wheat introgression lines produced through wide hybridization, 2 cultivated hexaploid wheat varieties, a tetraploid wheat variety and salt-tolerant accession D of Aegilops cylindrica. DNAs extracted from fresh leaves by the CTAB method were amplified using 25 µ.L reaction volume in a Perkin Elmer Thermal Cycler and randomly sequenced 10 mer synthetic primers. Based on amplification reactions, all the primers were divided into 4 categories. Category A primers reacted with all the wheat lines, category B with none, category C with 10-14, and category D with 3-7 wheat lines. Of the 18 reactive primers, 11(61%) detected polymorphism in all the test material. The level of polymorphism was low and ranged from 1.2 to 22.8%. Most of the primers produced monomorphic bands including very intense, easily visible and less visible bands. Of the 9 salt-tolerant introgression wheat lines, 6 were identified on the basis of polymorphic bands. Our study indicated possibilities for using Randon Amplified Polymorphic , DNA (RAPD) markers to detect specific variations in the genome that could be used for varietal finger printing.

Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan.