| Pak. J. Bot., 32(1): 45-56, 2000. | Back to Contents | ||||
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Updated: 08-04-11 | ||||
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QUICK DETECTION OF ASCOCHYTA LENTIS FROM LENTIL SEEDS USING POLYMERASE CHAIN REACTION (PCR) BASED TECHNIQUES SHAUKAT HUSSAIN*., TAKAO TSUKIBOSHI AND TSUTOMU UEMATSU
Abstract: Two Polymerase Chain Reaction (PCR) specific primers,
ASCO1 and ASCO2 were developed for the detection of Ascochyta lentis from
infected lentil seeds. PCR amplification with primers ASCOI and ASCO2 resulted
in amplification of an approximately 400bp product with A. lentis and A.
rabiei isolates but not with A. fabae and other lentil pathogens viz.
, Sclerotinia sclerotiorum, Stemphylium botryosum and Colletotrichum
lindemuthianum. The specific primers were amplified with the whole DNA of
the A. lentis infected seeds but not with healthy seeds suggesting that
the specific primers will provide a valuable tool for accurate and quick detection
of A. lentis in lentil seeds. National Institute of Agro-Environmental Sciences, 3-1-1, Kannondai, Tsukuba City, Japan. Permanent address - Pulses Program, National Agricultural Research Center, Islamabad 45500, Pakistan. |
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