| Pak. J. Bot., 32(1): 57-64, 2000. | Back to Contents | ||||
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Updated: 08-04-11 | ||||
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TRANSFER AND BEHAVIOUR OF SOME R PLASMIDS IN PLANT ASSOCIATED BACTERIA FASIHUDDIN AHMED ANSARI, MUHAMMAD TAUSEEFUR REHMAN, NAYYAR JAHAN AND HAJRA KHATOON
Abstract:
Eleven R plasmids isolated
from enteric bacteria were studied for their transfer and stability in plant associated
bacteria to use them as vehicles for In vivo genetic engineering. The bacteria
of plant origin viz., Xanthomonas, Erwinia, Citrobacter, Rhizobium, Agrobacterium
and Pseudomonas used as recipients and the R plasmids used included pFK2,
pFK6, pFK7, pFK8, pFK20, pAS72, pNJ15, pNJ18, pFK5a, pFK17 and pFK18. The R plasmids
which carried resistance to one or five different antibiotics were tested for
their conjugal transmission from enteric bacteria to the bacteria of plant origin.
Two of these R plasmids viz., pFK6 and pFK7 could be conjugally transmitted to
four different bacteria of plant origin whereas, pFK2 could be transmitted to
three and the rest could be transmitted to two or one plant associated bacteria.
Most of the R plasmids were found to be stably maintained after their transmission
into the bacteria of plant origin indicating that they could be used as vehicles
for In vivo genetic engineering of these bacteria. Department of Microbiology, University of Karachi, Karachi-75270, Pakistan. |
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