| Pak. J. Bot., 33(1): 35-41, 2001. | Back to Contents | ||||
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Updated: 01-12-10 | ||||
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IN VITRO AND IN VIVO MULTIPLICATION OF VIRUS-FREE 'SPUNTA' POTATO RIDA A. SHIBLI, ABDULLAH M. ABU-EIN AND MOHAMMED M. AJLOUNI Abstract: In vitro shoots of Solanum tuberosum cv 'Spunta' were subcultured on liquid MS media containing 0.0, 0.5, 1.0, 1.5 and 2.0 mg/l of benzyl adenine (BA) or kinetin. Significant reduction in stem and internode length was observed by increasing BA and kinetin concentrations. BA up to 1.0 and 1.5 mg/l showed an increase in number of proliferated shoots and number of nodes per flask. Single node cuttings were rooted on solid media containing NAA, IBA, or IAA @ 0.0, 0.5, 1.0, 1.5, 2.0 mg/l, individually with or without 1.0 mg/l GA3. Sucrose concentration from 30 to 90 g/l gave significant enhancement of microtuberization. In vitro rooted shoots were successfully acclimatized. Cuttings, 3.0 cm from glasshouse grown plants were successfully rooted by treating 0.5 cm base with 1 g/l IBA + 0.5 g/l IAA for five seconds. Plant Tissue Culture & Physiology Laboratory, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid-Jordan. |
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