Pak. J. Bot., 33(3): 257-266, 2001. | Back to Contents | ||||
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Updated: 01-12-10 | ||||
CHANGES OF PROTEINS AND OXIDATIVE ENZYMES IN SEEDS AND IN VITRO REGENERATED PLANTS OF THREE IRANIAN CULTIVARS OF WHEAT (TRITICUM AESTIVUM L.) KH. KIAROSTAMI AND H. EBRAHIMZADEH
Abstract: Somaclonal variation is one of the possible sources
of variation in plant breeding. To evaluate the usefulness of somaclonal
variation for creating variation in some Iranian cultivars of wheat,
changes in proteins and oxidative enzymes in regenerated and seed-produced
plants of wheat were studied. Three populations of each plant were used
each of which belong to one embroyogenic callus or one ear. Immature
embryos from three cultivars of wheat (Alamout, Hyrmand and Maroon),14
days after anthesis were cultured on solid MS medium with 1mgl-1
2,4-D. The embryogenic calli obtained were then transferred to solid
hormone-free MS medium to produce plantlets. Seeds of these three cultivars
of wheat were also cultured on solid hormone free MS medium. Plantlets
obtained from the two above sources were collected and used for the
study of proteins, peroxidases, polyphenoloxidases and superoxidedismutases.
There was no significant difference in the banding pattern SDS-PAGE
and PAGE systems.Comparison of proteins between different regenerated
or between different seed plants and the resulting dandrogram showed
that the plants derived from seed culture belong to one group and those
derived from tissue culture belong to another group. The level of peroxidase
isozymes were lower in regenerated plants while superoxidedismutase
represented additional isozyme in regenerated plants. Polyphenoloxidase
had equal isozymes in both kinds of plant. Department of Biology, Faculty of Science, Tehran University, Tehran, Iran. |
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