| Pak. J. Bot., 35(1): 13-16, 2003. | Back to Contents | ||||
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Updated: 16-05-12 | ||||
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ADVENTITIOUS SHOOT REGENERATION FROM LEAF EXPLANTS OF KAKROL CULTURED IN VITRO A. HOQUE AND S.M. RAHMAN
Abstract: Adventitious
shoot bud differentiation occurred from the petiolar cut ends of leaf explants
of Momordica dioica (Roxb.) Willd.. cultured on Murashige and Skoog medium
supplemented with different concentrations and combinations of BA and NAA. The
best response of shoot proliferation was obtained in medium supplemented with
2.0 mg/l BA and 0.2 mg/l NAA. Direct and indirect (via callus) shoot bud differentiation
were observed. After 3 weeks of culture, calli were transferred onto the MS medium
supplemented with aforesaid growth regulator for bud regeneration. Rooting was
induced with 1.0 mg/l BA and plantlets were survived successfully to field condition.
This is the first description of adventitious bud regeneration from leaf explants
of kakrol. Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh. |
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