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THE HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNIT COMPOSITION OF WHEAT (TRITICUM AESTIVUM L.) LANDRACES FROM PAKISTAN

RASHID ANWAR, SHAHID MASOOD, MIR AJAB KHAN AND S. NASIM

Abstract: The endosperm storage proteins of 127 wheat landraces collected from different parts of Pakistan were fractionated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to determine their high molecular weight (HMW) glutenin subunit composition. A total of 13 different subunits were found in the present set of wheat germplasm. Of these alleles detected at all the Glu-1 loci, three belong to Glu-A1, five each to Glu B1 and Glu D1 locus. Subunit null controlled by locus Glu-A1, 17+18 by Glu-B1 and 2+12 by Glu-D1, predominantly. Different accessions possessed between three and five HMW glutenin subunit. The frequency of alleles in the entire set of germplasm ranged from 0.79 to 80.31% which indicate a higher degree of genetic diversity. The quality score calculated according to Payne (1986) ranged between 4 and 10 in the landraces examined. The information generated in this study could be utilized to improve the bread or chapati making quality.
 

Plant Genetic Resources Institute, NARC, Islamabad, Pakistan.