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ISOLATION AND PARTIAL PURIFICATION OF ELICITOR MOLECULES FROM COLLETOTRICHUM LINDEMUTHIANUM

FATIMA BI* AND PAUL FINCH

Abstract: Colletotrichum lindemuthianum grown in glucose/neopeptone natural liquid medium showed that yield of crude 'High Molecular Weight Culture Filtrate Elicitor' (HMWCFE) obtained by simultaneous ultrafiltration and dialysis were little high 0.8 g/L in 6^th day culture. A range of browning was observed in response to various elicitor preparations of each day culture of C.lindemuthianum. Maximum browning was produced by the elicitor preparation in 6^th day culture. Total carbohydrate and protein contents were found to be 18.4 -33.4% and about 1.6-2.7% respectively. The presence of phosphate (4-12%) in high molecular weight culture filtrate elicitor is reported for the first time from C. lindemuthianum. Preliminary elution profile and apparent range of molecular weight of HMWCFE was >60 000 dalton determined by gel permeation chromatography.

Department of Chemistry, Royal Holloway Bedford New College, University of London, London, UK.

Author for correspondence: Pakistan Council of Scientific and Industrial Research Laboratories Complex, Karachi-75280, Pakistan.