Pak. J. Bot., 36(2): 321-329, 2004. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
IMPROVED BRASSICA NAPUS L., REGENERATION FROM HYPOCOTYLS USING THIDIAZURON AND BENZYLADENINE AS CYTOKININ SOURCES PARISSA JONOUBI, AMIR MOUSAVI,*, AHMAD MAJD1 AND JAHANFAR DANESHIAN2
Abstarct: A
reproducible system to produce regenerated Brassica napus L., plants has
been developed using hypocotyl explants. Different concentrations of Benzyladenine
(1.5, 3.0 and 4.5 mg l-1) and Thidiazuron (0, 0.15 and 0.30 mg l-1) were evaluated
for shoot regeneration using 7, 14 and 21 day old hypocotyl explants. Treatments
were arranged in a factorial experiment as a randomized complete block design
with three replications. Significant differences were found in the interactions
of Benzyladenine and Thidiazuron concentrations as well as the ages of the explants.
Maximum shoot regeneration was obtained using 4.5 mg l-1 Benzyladenine and 0.3
mg l-1Thidiazuron. Shoot regeneration was highly affected by the age of the explants.
It was found that 21 day old explants were more likely to undergo shoot development
than the others. Under these culture conditions, the highest percentage of shoot
regeneration was 174.0% for hypocotyl explants. Regenerated shoots rooted when
cultured on a root induction medium supplemented with 2 mg l-1 of Indolebutyric
acid. Rooted plantlets were successfully established in the soil which developed
normal fertile flowers and viable seeds. In light of its efficiency, this hypocotyl
regeneration method could be a suitable tool for genetic transformation using
Agrobacterium.
National Research Center
for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran. |
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