| Pak. J. Bot., 36(2): 439-444, 2004. | Back to Contents | ||||
|
|
Updated: 09-07-09 | ||||
|
DIVERSITY OF BIPOLARIS SOROKINIANA ISOLATES FROM WHEAT GROWING AREAS UNDER RICE-WHEAT CROPPING SYSTEM IN PUNJAB, PAKISTAN SHAZIA IRAM AND IFTIKHAR AHMAD
Abstract:
A survey was conducted for the assessment of foliar blight of wheat in
main rice-wheat cropping areas of the Punjab province, Pakistan. The
foliar fungus Bipolaris sorokiniana was isolated from leaf
samples. Isolates of B. sorokiniana were classified according to
their aggressive behavior based on disease severity scale. B.
sorokiniana isolates showed foliar blight symptoms on wheat but not
on rice. Random Amplified Polymorphism DNA (RAPD) was used to study the
genetic variation within the populations of the fungus. A correlation
study was carried out with the help of five primers viz., P1
(5´-AGGAGGACCC-3´), P2 (5´-ACGAGGGACT-3´), PE7 (5´-AGATGCAGCC-3´), P14
(5´-CCACAGCACG-3´) and PE20 (5´-AACGGTGACC-3´). A tree was constructed
based on the pattern of bands which highlighted the correlation between
morphological, aggressiveness and genetic variations of B.
sorokiniana. Institute of Plant & Environmental Protection (IPEP), National Agricultural Research Center, Islamabad, Pakistan. |
|||||
| Back to Contents |
|
Back to Contents | |||