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EFFECT OF SOME GROWTH HORMONES (GA₃, IAA AND KINETIN) ON THE MORPHOLOGY AND EARLY OR DELAYED INITIATION OF BUD OF LENTIL (LENS CULINARIS MEDIK)

M. NAEEM, IRAM BHATTI*, RAZA HAFEEZ AHMAD* AND M. YASIN ASHRAF**

Abstract: The effect of growth hormones on the morphology of shoot of lentil was examined. The hormones viz., 1.5 mM (500 mg L^-1) GA₃, 2.85 mM (500 mg L^-1) IAA and 0.14 mM (30 mg L^-1) kinetin were applied individually as well as in combination i.e., 1.5 mM GA₃ + 2.85 mM IAA, 1.5 mM GA₃ + 0.14 mM kinetin, 2.85 mM IAA + 0.14 mM kinetin and 1.5 mM GA₃ + 2.85 mM IAA + 0.14 mM kinetin. GA₃ showed a marked elongation in the length of shoot and increase in the number of internodes and compound leaves. Application of IAA showed a decrease in length of shoot and number of internodes. The increase in the diameter, area and number of leaves was also observed. IAA induced branching with lush green colour of leaves. Kinetin showed inhibition in length and in the number of internodes. Inhibition was associated with a significant expansion in diameter and an increase in area of leaves as well as their number. The combined dose of GA₃+IAA, GA₃+kinetin and GA₃+IAA+kinetin showed a significant increase in length and number of internodes as well as in the number of compound leaves. The colour of leaves was green and no branching was induced. However, the diameter of main stem showed inhibition. The dose of IAA+kinetin showed a decrease in length and number of internodes. However, expansion in the main stem diameter and increase in the number and area of leaves was also observed. The colour of leaves was lush green with more branches as compared to control. In GA₃ treated plants, early flowering with higher number of floral buds was recorded. Applied IAA caused late flowering and increased the number of floral buds, while kinetin showed no significant delay in flowering but number of floral buds was more as compared to control. The mixed doses of GA₃ with IAA and kinetin revealed early flowering alongwith nonsignificant increase in the number of flower buds. However, the dose of IAA+kinetin promoted late flowering with noticeable increase in number of floral buds.
 

Department of Botany, Government College Jaranwala,Faisalabad, Pakistan.
Institute of environmental Biology, Punjab University, Lahore, Pakistan.
Nuclear Institute for Agriculture and Biology, P.O. Box 128, Jhang Road, Faisalabad, Pakistan.