| Pak. J. Bot., 36(4): 817-823, 2004. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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IN VITRO SHOOT TIP CULTURE OF COTTON (GOSSYPIUM HIRSUTUM) BUSHRA RASHID*, TAYYAB HUSNAIN AND SHEIKH RIAZUDDIN
Abstract: Shoot apices from in-vitro germinated seedlings of 22 cotton
varieties were cultured on basal MS salts and vitamins (Murashige &
Skoog, 1962). No phytohomones were used. Growth response of different
varieties varied. Root and shoot formation, was observed in all varieties.
After plantlet formation, in vitro plantlets were shifted to
pots containing a specific composition of soil, sand and peat moss.
Pots were covered with polythene bags to maintain the humidity level
and uncovered for a limited period for aeration for three weeks. These
plants grew into healthy green plants and reached to maturity. Centre for Applied Molecular Biology, 87-West Canal Bank Road, Thokar Niaz Baig Lahore-53700, Pakistan. Corresponding Author: bushra_2002@lycos.co.uk Tel: 042-5423942-45, Fax: 042-5421316 |
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