| Pak. J. Bot., 36(4): 835-843, 2004. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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GENETIC DIVERSITY IN WHEAT LANDRACES FROM PAKISTAN BASED ON POLYMORPHISM FOR HIGH MOLECULAR WEIGHT GLUTENIN SUBUNITS (HMW-GS) M. SHAHID MASOOD, M. ASGHAR AND RASHID ANWAR Abstract: A total of 77 wheat (Triticum aestivum L.) landraces collected
from different areas of Pakistan were assessed for genetic diversity
in terms of HMW-Glutenin sub-units. Considerable amount of variation
was observed in this set of germplasm ranging from monomorphism to polymorphism
as revealed by SDS-Polyacrylamide gel electrophoresis (SDS~PAGE). Of
the 8 alleles detected at Glu-1 loci, three belong to each Glu-A1 and
Glu-B1 and 2 belong to Glu-D1 locus. The most frequent subunits were
1 and 2* at Glu-A1 locus, 7+8 at Glu-B1 locus and 2+12 at Glu-D1 locus.
Maximum variation was observed in Diamir followed by Ghizer. A higher
gene diversity between the populations (Dst = 0.29%) was observed as
compared to the gene diversity within the populations (Hs =10%). High
value for gene diversity between population relative to total gene diversity
(Gst = 0.7376) indicated a substantial amount of gene differentiation
among the populations. The information generated could be exploited
by the plant breeders to develop new cultivars and by the gene bank
managers to properly document and maintain the germplasm collections.
Plant Genetics Resources Institute, NARC Islamabad, Pakistan. |
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