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BIOCHEMISTRY OF RESISTANCE IN CHICKPEA AGAINST WILT DISEASE CAUSED BY FUSARIUM OXYSPORUM F. SP. CICERIS

IFTIKHAR A. KHAN, S. SARWAR ALAM, A. HAQ AND ABDUL JABBAR*

Abstract: Chickpea lines Flip 90-131C, Flip 96-152C, Flip 96-153C, Flip 96-155C, Flip 96-158C and ICCV 95503 were found highly resistant (0% incidence) to wilt disease whereas Flip 85-29C, Flip 85-30C and Flip 96-154C (16-17% incidence) were resistant to wilt disease. The chickpea lines Flip 85-29C, Flip 89-14C, Flip 90-2C, Flip 92-148C and UC 27 were found resistant when screened against culture filtrate of the same isolate, while the lines Flip 90-74C, Flip 96-153, Flip 96-155C, Flip 96-157C, ICCV 95503 and UC 15 were tolerant. The two methods of screening did not show complete correlation. Total phenols in the uninoculated roots of resistant/susceptible test lines did not show any correlation with the wilt resistance because the susceptible lines produced higher phenolic contents as compared to the resistant lines. The uninoculated roots of resistant chickpea lines produced antifungal compounds whereas the susceptible line did not produce any active compounds. The inoculated roots of both resistant and susceptible lines produced higher antifungal activity as compared to uninoculated ones. The resistant chickpea lines produced an additional antifungal compound at Rf value 0.79 which was absent in susceptible lines, which might have a role in imparting resistance against wilt disease. The methanol extract of the stem produced one inhibitory zone at Rf value 0.11.
 

Nuclear Institute for Agriculture and Biology, PO Box 128 Jhang Road Faisalabad, Pakistan.
Department of Chemistry, Islamia University, Bahawalpur, Pakistan.