Pak. J. Bot., 37(2): 263-269, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
USE OF SDS-PAGE MARKERS FOR DETERMINING QUANTITATIVE TRAITS LOCI IN BLACKGRAM [VIGNA MUNGO (L.) HEPPER] GERMPLASM ABDUL GHAFOOR*, ZAHOOR AHMAD AND MUHAMMAD AFZAL
Abstract:
One hundred and five genotypes of blackgram from diverse origin were
evaluated for agronomic traits for 2 years and seed proteins were analyzed
using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
through vertical slab type unit. Screening analysis for markers to quantitative
traits revealed its significance in determining quantitative trait loci
(QTL) in blackgram through SDS-PAGE markers. The factors affecting quantitative
traits may occur as individual genes or gene clusters scattered throughout
the genome, therefore, quantitative traits were expected differently
at several loci. Variation in seven quantitative traits out of ten was
significantly associated with 9 protein sub-units, however, the actual
number of QTLs might be fewer because several of these traits were correlated.
Variation at protein peptides in the vicinity of QTL in blackgram may
be an indication of genetic variation potentially available to breeding
programmes. Expansion of genetic base for blackgram breeding might be
accomplished by systematic use of germplasm that differ from common
banding pattern and known to be associated with variation in quantitative
traits. Plant Genetic Resources Institute (PGRI), National Agricultural Research Centre (NARC), Islamabad, Pakistan. Corresponding Author: ghafoor59@hotmail.com Phone 92-51-9255203, Fax 92-51-9255201 |
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