Pak. J. Bot., 37(2): 271-277, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
DNA FINGERPRINTING STUDIES OF SOME WHEAT (TRITICUM AESTIVUM L.) GENOTYPES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS MUHAMMAD ASIF, MEHBOOB-UR-RAHMAN AND YUSUF ZAFAR*
Abstract:
Random amplified polymorphic DNA (RAPD) analysis was employed to estimate
genetic diversity/genetic similarity among nine wheat (Triticum aestivum
L.) genotypes. Out of 25 random 10-mer primers surveyed, 18 primers
detected polymorphism among all the wheat genotypes, while 7 primers
produced monomorphic pattern. A total of 175 bands were amplified, 60.57%
of which were polymorphic. Genetic similarity matrix was generated on
the basis of Nei and Li's coefficients whose values ranged from 61.29%
to 88.03%. The coefficients were used to make clusters using unweighted
pair group method of arithmetic means (UPGMA). Wheat genotypes were
grouped in two main clusters (A and B). However, CMH-771917/PKV1600
and VEE/Trap # 1 were the most distant genotypes, which were 61.29 to
76.53% and 66.01 to 78.81% genetically similar with the other genotypes,
respectively. The information generated here along with the use of most
advanced DNA fingerprinting techniques like AFLP, SCAR, SSR and ESTs
would be helpful for future genome mapping programs as well as for the
application of intellectual breeder rights in the country. The study
will also work as indicator for wheat breeders to evolve varieties with
diverse genetic background to achieve sustainability in wheat production
in the country. National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan Corresponding Author: y_zafar@yahoo.com Fax: +92-41-651472 |
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