Pak. J. Bot., 37(4): 823-828, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
TISSUE CULTURE STUDIES IN ORYZA SATIVA L. CVS. BASMATI 385 AND SUPER BASMATI S. M. SAQLAN NAQVI, RAZIA SULTANA AND HAMID RASHEED*
Abstract: The main objective of the present study was to investigate and optimize
callus induction frequency and regeneration in two rice cultivars viz.,
Basmati 385 and Super basmati and to monitor the effect of mannitol
and sorbitol on regeneration. Callus induction was achieved by using
different concentrations of 2, 4-D (1, 2, 3, 4 mgL-1) on
MS and N6 media with 2 mgL-1producing the best results. Regeneration
was studied only on MS medium containing BAP, NAA and 3% sucrose with
or without either 3% mannitol or sorbitol. Super Basmati showed better
results both for callusing and regeneration. Regeneration frequency
decreased by the addition of either mannitol or sorbitol.
Department of Biochemistry, University of Arid Agriculture Rawalpindi,
Pakistan. |
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