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EFFECT OF GROWTH HORMONES ON SHOOT PROLIFERATION OF ROSE CULTIVARS

M. JAFAR JASKANI, M. QASIM, JAVERIA SHERANI¹, ZAHOOR HUSSAIN AND HAIDER ABBAS²

Abstract: Roses are commonly propagated asexually but the conventional methods are tedious and time consuming with low percentage of success. Tissue culture technique has been proved as a potential tool for quick and mass propagation of several plant species. The research was conducted to explore the optimum levels of Kinetin, NAA, IAA and BA supplemented in MS medium for micropropagation of rose cvs. Queen Elizabeth and Angel Face. Maximum shoot initiation percentage (80%) was achieved with BAP 3.0 mgL^-1 + Kinetin 3.0 mgL^-1. Shoot length was maximum (3.0 cm) on MS medium with IAA (3.0 mgL^-1) and BAP 0.3 mgL^-1 BAP while on MS medium added with BAP 3.0 mgL^-1 + Kinetin 3.0 mgL^-1 shoots attained the length of 1.5 cm. It was also observed that young shoot tips as an experiment performed better than mature shoot tips. Overall performance of Queen Elizabeth was better than Angel Face cultivar.
 

Institute of Horticultural Sciences, University of Agriculture, Faisalabad-38040, Pakistan.
College of Agriculture, D.G. Khan, University of Agriculture, Faisalabad-38040, Pakistan.
Department of Botany, University of Karachi, Karachi, Pakistan.