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MORPHOGENIC POTENTIAL OF THREE POTATO (SOLANUM TUBEROSUM) CULTIVARS FROM DIVERSE EXPLANTS, A PREREQUISITE IN GENETIC MANIPULATION

IQBAL HUSSAIN, AISH MUHAMMAD, ZUBEDA CHAUDHRY, REHANA ASGHAR*, S.M. SAQLAN NAQVI** AND HAMID RASHID#

Abstract: In vitro response and its relationship with different varieties, explants and media were investigated in potato (Solanum tuberosum). Direct In vitro regeneration protocol from diverse explant source is a prerequisite for transformation studies. Three potato cultivars viz., Cardinal, Altamash and Diamont were selected for in vitro responses. High regeneration and morphogenic potential of different explants i.e., shoot tips, leaf discs, nodes and internodes have been tested for direct regeneration. Basal media was Murashige & Skoog and different hormonal combinations of benzyl adenine and indoleacetic acid were supplemented. Statistical analysis showed that explant source had significant effect on direct regeneration and the nodal explants had maximum regeneration. The number of shoots obtained from node was 17.6 from Cardinal followed by Diamont 14.3 and Altamash 9.0. Shoot apices also resulted in shoot regeneration comparatively better than leaf discs and internodal explants but lesser than from nodes. Most suitable medium was MS with 2.0 mg/l BAP and IAA @ 0.5 mg/l giving maximum regeneration. It was also observed that interaction of cultivars with explant and media is highly significant at P 1.0%.
 

Agriculture Biotechnology Programme (IABGR) NARC, Islamabad, Pakistan.

Department of Botany, University of Arid Agriculture Rawalpindi, Pakistan
Department of Biochemistry, University of Arid Agriculture Rawalpindi, Pakistan
Corresponding Author: Iqbal_abi@yahoo.com