| Pak. J. Bot., 37(4): 981-987, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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EFFECT OF LOW pH ON CONTINUOUS CITRIC ACID FERMENTATION BY ASPERGILLUS NIGER SIKANDER ALI, IKRAM-UL-HAQ AND JAVED IQBAL
Abstract: The present study is concerned with the effect of low pH on continuous
citric acid fermentation by Aspergillus niger strain SK-17. Microbial
cultivations were carried out in a stainless steel stirred bioreactor
of 15 L total capacity. The bioconversion of glucose to citric acid
at low pH was investigated on cellulose fabric. Sucrose solution (100
g/l) was made to flow through capillaries of a vertical fabric support
and oxidized to citric acid at the interface. Conditions of temperature,
humidity, airflow and glucose feed rate were optimised. The emerging
broth contained a product concentration of 120-140 g/l of citric acid,
which is higher than the expected [maximum of 109 g citric acid per
100 g sucrose] as a result of evaporative concentration during the downward
flow. Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan. |
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