Pak. J. Bot., 37(4): 989-995, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
INDUCTION OF SYSTEMIC RESISTANCE IN CHICKPEA AGAINST FUSARIUM WILT BY SEED TREATMENT WITH SALICYLIC ACID AND BION NIGHAT SARWAR, M. HAYAT ZAHID CH., IKRAMUL HAQ, AND F. F. JAMIL
Abstract: Seeds of chickpea variety AUG424, susceptible to Fusarium oxysporum
ciceri (FOC), were surface sterilized with sodium hypochlorite,
thoroughly rinsed with sterilized water and immersed in two concentrations
of Salicylic acid (1.0 & 1.5 mM) and Bion (0.3& 0.4mM). Seeds
treated with 2% Benlate were used as standard. Control represented,
the seeds soaked in distilled water. Control and treated seeds were
sown in two sets of pots containing sterilized soil (experiment 1) and
soil inoculated with FOC (experiment 2). Chemically treated and control
seeds were grown under controlled environment. Two week old seedlings
grown in sterilized soil were up rooted, roots were cut at 1cm from
tip and immersed in spore suspension (106 micro conidia/ml) of FOC for
three hours and then the seedlings were transplanted into new pots containing
sterilized soil. Plants of both sets were observed daily for up to 40
days to record wilt disease by counting the total and wilted plants
in each pot. At the end of the experiment, surviving plants were cut
at collar region for observing the fungus growth inside the vascular
tissues. Fresh and dry weight of the shoots and roots were recorded.
Wilt disease was significantly reduced with all the treatments in both
experiments. On the basis of disease rating done after root cutting,
wilt incidence was significantly less in chemically treated plants as
compared to control ones. Fresh and dry weights of shoot and root were
higher in treated plants as compared to control ones especially in plants
grown from Bion treated seeds. Nuclear Institute for Agriculture and Biology, P. O. Box 128, Jhang Road, Faisalabad, Pakistan. |
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