| Pak. J. Bot., 38(2): 267-274, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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COMPARISON OF THE EFFECTS OF DIFFERENT SA CONCENTRATIONS ON SENESCENCE IN EXCISED ROCKET (ERUCA SATIVA L.) COTYLEDONS GÜL CEVAHIR*, SERAP ÇAĞ, SEMAHAT YENTÜR, MUAMMER ÜNAL, ELIF AYTAMKA, AND ÇIGDEM SELÇUKCAN*
Abstract: Senescence, accepted as the last phase of growth in plants, is characterized
by a series of degenerative events that decrease the metabolic activity
and eventually cause the death of cells, tissues and organs. In the
present studies the effect of salicylic acid, a phenolic substance that
recently came to be defined as plant growth regulator, especially increasing
immunity of plants against infections, regulating thermogenesis in Arum
flowers, inhibiting ethylene biosynthesis and germination, reversing
the effects of abscisic acid and blocking the wound-induced response
on the senescence of excised cotyledons of cultivated Eruca sativa
L., (Rocket) was examined. Cotyledons of ten-day-seedlings of E.
sativa were cut apart from the petiole and used as experiment materials.
The fresh weight of cut cotyledons were measured and they were then
incubated in 3 ml of salicylic acid at 10-3, 10-5, 10-7, 10-9 M concentrations
and in distilled water as control. After 4 hours of light exposure in
climatie room (25 2 C), total chlorophyll amount, total protein content,
peroxidase activity and protein analysis with SDS-PAGE method were assayed
spectrophotometrically with biochemical methods. Furthermore, the effect
of salicylic acid on fresh weights of rocket cotyledons was studied.
Department of Botany, Faculty of Science, Istanbul University, 34134 Süleymaniye, Istanbul, Turkey.
Istanbul University, Informatics Department, Vezneciler Istanbul,
Turkey. |
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