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EFFECT OF CHLOROCHOLINE CHLORIDE, SUCROSE AND BAP ON IN VITRO TUBERIZATION IN POTATO (SOLANUM TUBEROSUM L. CV.CARDINAL)

IQBAL HUSSAIN, ZUBEDA CHAUDHRY, AISH MUHAMMAD, REHANA ASGHAR*, S.M. SAQLAN NAQVI* AND HAMID RASHID**

Abstract: Protocols for in vitro tuberization of potato (Solanum tuberosum L.) variety Cardinal were studied. Virus free cultures were multiplied on Murashige & Skoog (1962) medium containing 1.0 mg/l GA₃. When the number of shoots was maximum the cultures were shifted on tuber induction medium. In vitro tuberization media consisted of MS along with different concentrations of CCC, BAP and Sucrose. It was observed that 200 mg/l of CCC or 90g/l sucrose in the mediums resulted in maximum tuber induction (16.5 and 15.6 tubers/flask respectively) followed by BAP @ 4.0mg/l producing 9.0 tubers/flask. It was also observed that complete darkness was essential for tuber induction.
 

Agricultural Biotechnology Program (IABGR) NARC, Islamabad, Pakistan.

* University of Arid Agriculture, Rawalpindi, Pakistan.
** Corresponding Author: Iqbal_abi@yahoo.com