| Pak. J. Bot., 38(2): 385-391, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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EFFECT OF DIVERSE HORMONAL REGIMES ON IN VITRO GROWTH OF GRAPE GERMPLASM GHULAM MUSTAFA SAJID*, MUHAMMAD KASHIF ILYAS AND RASHID ANWAR
Abstract:
Two accessions of grape germplasm were tested on Murashige-Skoog media
containing varying levels of BAP either or not containing IAA and NAA
for in vitro growth parameters. It was observed that shoot number
and shoot mass increased when the concentration of BAP was increased
in a linear fashion. The shoot length of both the accessions showed
a downward trend when the concentration of BAP was increased. The presence
of IAA or NAA in the media, however, had a positive effect on the shoot
length even in the presence of BAP (0.2 mg/l). It was possible to induce
root induction on hormone free media for both the accessions used but
the presence of NAA or IAA in the media had a profound effect on root
induction of both the accessions. NAA was found to be more effective
in root induction than IAA. Increased levels of BAP were found to inhibit
root mass accumulation in both the accessions but the degree of inhibition
was found to be accession specific.
Institute of Agricultural Biotechnology and Genetic Resources (IABGR),
National Agricultural Research Center (NARC), Islamabad, Pakistan. |
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