| Pak. J. Bot., 38(3): 583-587, 2006. | Back to Contents | ||||
|
|
Updated: 09-07-09 | ||||
|
PROPAGATION OF CTV-FREE SWEET ORANGE [CITRUS SINENSIS (L.) OSBECK] PLANTS THROUGH MICROBUDDING TECHNIQUE MAZHAR ABBAS, M. M. KHAN,*, S. M. MUGHAL1, MUHAMMAD J. JASKANI1 AND HAIDER ABBAS2
Abstract: Microbudding technique was used for the early propagation of sweet
orange [Citrus sinensis (L.) Osbeck] cv. Musambi plants, free
of citrus tristeza virus (CTV) using thermotherapy. Source plants of
sweet orange, both infected and apparently healthy, were collected from
the orchards at Sahiwal and Faisalabad and analyzed along with microbudded
plants through ELISA. Budwood of 3 mm and 4 mm in size was microbudded
at two different heights (15 cm and 23 cm). Maximum (50%) success of
microbudding was obtained, when budwood of 4mm was microbudded at the
height of 23 cm. It would suggest that microbudding could efficiently
be used for the early propagation of CTV free Musambi plants. Citrus Nursery Sanitation Lab., Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan.
1 Department of Plant Pathology, University of Agriculture, Faisalabad,
Pakistan. |
|||||
| Back to Contents |
|
Back to Contents | |||