| Pak. J. Bot., 38(3): 589-595, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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EMBRYOGENESIS IN UNDEVELOPED OVULES OF CITRUS CULTIVARS IN RESPONSE TO GAMMA RADIATION NAFEES ALTAF
Abstract: Immature fruits of 36 Citrus cultivars were exposed to 0 - 12 kr gamma
radiation. The maximum average embryo per nucellus (EN) were recorded
after 4 months of ovule culture. In control (0 kr), the EN were 6.27,
6.06, 5.62 and 4.86 in Kinnow, Jaffa, Gada dehi and Sweet lime respectively.
In 3 kr, EN were 6.85, 6. 23, 5.86, 4.60 in Foster, Kinnow, Valencia
and Gada dehi, respectively. In 6 kr, EN were 8.33, 8.12, 5.66 and 3.66
in Foster, Kinnow, Pine apple and Kherna Khatta respectively. In 9 kr,
EN were 7.31, 6.76, 5.30 and 3.15 in Kinnow, Foster, Jaffa and Kherna
Khatta respectively. In 12 kr, EN were 5.88, 3.92, 3.80 and 2.50 in
Kinnow, Foster, Jaffa and Rough lemon respectively. In general, there
was suppression of embryogenesis in 9 and 12 kr. The healthy embryos
with normal balanced germination were ex-vitro grafted on rough lemon
seedlings for growth and development. Nuclear Institute for Agriculture & Biology (NIAB) P.O. Box 128, Faisalabad, Pakistan. |
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