| Pak. J. Bot., 38(3): 597-601, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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IN VITRO BUD CULTURE OF KINNOW TREE NAFEES ALTAF
Abstract: Tissues from field grown trees have contamination problems in In vitro
cultures. Two hundred explants were tried in each sterilizing chemical
viz., HgCl2 and NaOCl. Both the chemicals were effective in making clean
explants growth as 62 (31%) and 67 (33%) respectively. The dead explants
were 25 and 23. The buds sprouted and upon subculturing, shoots were
grown in MS medium supplemented with BA (2 mg/l), GA (1 mg/l), proline
(5 mg/l). The developed shoots rooted in half strength medium with addition
of IBA (2 mg/l). Our objective was to obtain rooted shoots. Although
there were callus formation, leaf growth but no true shoot elongation.
The rooted shoots were grafted on young rough lemon seedlings. The overall
procedure is lengthy but plants can be obtained from In vitro bud culture
of field grown Kinnow tree. Nuclear Institute for Agriculture & Biology (NIAB) P.O. Box 128, Faisalabad, Pakistan. |
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