| Pak. J. Bot., 38(3): 631-636, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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PLANT REGENERATION VIA ORGANOGENESIS OR SOMATIC EMBRYOGENESIS IN SUGARCANE: HISTOLOGICAL STUDIES IMTIAZ AHMED KHAN AND ABDULLAH KHATRI
Abstract: Embryogenic callus was obtained by culturing young leaves of sugarcane
on modified MS medium containing 4mg/l 2,4-D. Many embryos were formed
when the callus was transferred on the medium containing low concentration
of 2,4-D (0.5 mg/l). The callus transferred on the medium without 2,4-D
turned black after 7 to 10 days of sub-culturing. Embryos were cytoplasmically
rich and showed the typical organization of grass embryos. Somatic embryos
were regenerated on the regeneration media (MS salts + 2 mg/l each Kin,
IAA and IBA + 2% sugar) and profused rooting was observed on medium
containing MS + 1 mg/l IBA + 6% sugar. Rooted plantlets were transferred
into the field after hardening for further evaluation. Agriculture Biotechnology Division Nuclear Institute of Agriculture, Tando Jam, Pakistan. |
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