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CALLUS INDUCTION AND PLANT REGENERATION FROM MATURE EMBRYOS OF DIFFERENT WHEAT GENOTYPES

AYŞE GUL NASIRCILAR, KENAN TURGUT*, KAYAHAN FISKIN

Abstract: Mature embryos of 5 Triticum aestivum and 5 T. durum cultivars formed embryogenic callus on two different media. Embryos were removed from surface sterilised seeds and placed with the scutellum upwards on a solid agar medium containing the inorganic components of Murashige & Skoog and 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg/L naphtalenacetic acid (NAA). The developed calli and regenerated plants were maintained on 2,4-D or NAA free MS medium. Wheat plants can be regenerated via two different systems. There were significant differences in percentage of callus induction and regeneration capacity on the different initiation medium. Among the T.aestivum cultivars, Yakar had the highest regeneration capacity in both induction medium. In T.durum cultivars, Kiziltan gave the highest regeneration capacity in MS+2,4 D medium and Yilmaz gave the highest regeneration capacity in MS+NAA medium. A strong genotypic effect on the culture responses was found for both induction medium.
 

Department of Biology, Faculty of Science & Arts, Akdeniz University, Antalya, Turkey.

Department of Crop Fields, Faculty of Agriculture, Akdeniz University, Antalya, Turkey.