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EFFECT OF MEDIUM, TEMPERATURE, LIGHT AND INORGANIC FERTILIZERS ON IN VITRO GROWTH AND SPORULATION OF LASIODIPLODIA THEOBROMAE ISOLATED FROM MANGO

MUHAMMAD ALI KHANZADA, ABDUL QAYOOM RAJPUT AND SALEEM SHAHZAD

Abstract: In this study the effects of culture media, temperature, light and fertilizers on mycelial growth and pycnidial production by Lasiodiplodia theobromae were evaluated. Potato sucrose agar (PSA), Corn meal agar (CMDA) and Yeast extract manitol agar (YEMA) were most suitable for mycelial growth of the test fungus. Potato carrot agar (PCA) was not suitable for either mycelial growth or pycnidia production. The YEMA appeared to be the best medium for pycnidial formation. The fungus grew from 20 to 45ºC, with optimum growth at 30-40ºC with no growth below 15ºC. The maximum numbers of pycnidia were produced at 35-40ºC. Different light regimes had no impact on mycelium growth and pycnidia production. The increasing concentrations of urea and DAP reduced the mycelial growth and pycnidia production.

Pest & Disease Research Lab., Department of Agriculture, University of Karachi, Karachi-75270, Pakistan.