Back to Contents

FREE FULL TEXT PDF

FACILE PLANT REGENERATION FROM TOMATO LEAVES INDUCED WITH SPECTINOMYCIN

MUHAMMAD SARWAR KHAN*, MUHAMMAD USMAN AND MUHAMMAD ILYAS LILLA

Abstract: Several studies have been conducted to find a combination of hormones conducive to improve regeneration from different explant tissues, particularly from leaves of tomato, and various responses were recorded to use in experiments. Major focus was to regenerate maximum number of shoots from tomato leaves Cv. Moneymaker, Packet, Nagina and Aroma. Depending on the cultivar used, shoot regeneration varied (Moneymaker = Packet = Nagina = Aroma) on RMOT medium (MS salts supplemented with 1mg/L Zeatin and 1mg/L IAA). In addition, several other media were used to compare regeneration efficiencies including RMOP medium containing MS salts supplemented with 0.1mg/L NAA, 1mg/L BAP, 1mg/L thiamine and 100mg/L Myo-inositol. In cultures where negative factors were eliminated, shoot regeneration reached to an average of 9 plants on RMOT medium from each leaf section of 3x3 mm size of cultivar Moneymaker.

National Institute for Biotechnology and Genetic Engineering (NIBGE), 577 Jhang Road, Faisalabad, Pakistan.

Corresponding Author: sarwarkhan_40@nibge.org