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EFFECT OF SUCROSE AND GROWTH REGULATORS ON THE MICROPROPAGATION OF SUGARCANE CLONES

IMTIAZ AHMED KHAN**, *M. UMAR DAHOT, SHAFQAT YASMIN, ABDULLAH KHATRI, NIGHAT SEEMA AND MAZHAR H. NAQVI

Abstract: The rapid multiplication of early, mid and late maturing sugarcane clones were obtained under different concentrations of commercial sugar and plant growth regulators in this study. Late maturing sugarcane clone AEC82-223 did not produce any auxillary shoot at 6% commercial sugar concentration. In contrast multiplication rate was higher in early and mid maturing sugarcane clones NIA-2004, BL4 and NIA-98, respectively. Apical meristems were cultured on modified MS medium containing different concentrations of auxins and cytokinins. An optimal multiplication was observed on M4 (1.0 mg/1 IAA + 1.0 mg/1 BAP + 0.1mg/l Kinetin) and M5 (0.l mg/1 kinetin + 1.5mg/l BAP + 1.5mg/l IAA). Maximum numbers of shoot were observed in BL4 followed by NIA-2004 when 1.0 mg/l of BAP and IAA were applied with low concentration of kinetin (0.1 mg/l). Shoot elongation and multiplication was improved on media devoid of BAP (MS+ 2 mg/1 kinetin + 2 mg/1 IBA + 2 mg/1 IAA). Best rooting was observed on media containing MS+ 1 mg/1 IBA + 6% sucrose.

Agriculture Biotechnology Division, Nuclear Institute of Agriculture, Tando Jam, 70060, Sindh, Pakistan.

Institute of Biotechnology and Genetic Engineering, Sindh University, Jamshoro, Pakistan.

Corresponding Author: Imtiaz Ahmed Khan; imtiaz_khan2@hotmail.com