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EFFICIENT EMBRYOGENIC SYSTEM FROM TISSUE CULTURE OF MATURE EMBRYOS FOR SOME COARSE VARIETIES OF RICE (ORYZA SATIVA L.)

S.M. SAQLAN NAQVI**, TASAWAR SULTANA, TAYYABA YASMIN, TARIQ MAHMOOD AND M. SHAHEEN AKHTAR*

Abstract: Five coarse cultivars of rice (Oryza sativa L.) viz., Pakhal, Swat-1, JP-5, KS-282 and IR-6 were evaluated for their ability to undergo somatic embryogenesis. Results indicated that N6 basal medium supplemented with 2 mg/L of 2, 4-D without BAP was the most appropriate for callus growth but inferior in its regeneration potential. Among all the genotype tested JP-5 showed highest 84 %) whereas IR-6 showed the lowest (63 %) callus induction frequency. (Regeneration rate was highest (82 %) on N6 basal media supplemented with 5 mg/L of BAP and 0.5 mg/L of IAA. All of the varieties showed a good regeneration potential on culturing, the highest for KS-282 and Pakhal while JP-5 showing the lowest regeneration compared with other genotypes studies. It was observed that sucrose supported regeneration better than sorbitol and mannitol. Calli on sucrose containing media showed higher regeneration and produced longer shoots whereas mannitol could not support the growth.

Department of Biochemistry, University of Arid Agriculture, Rawalpindi.

Biology Department, F.G. Degree College, H/9, Islamabad, Pakistan

Corresponding Author: saqlan@uaar.edu.pk