| Pak. J. Bot., 38(4): 1015-1026, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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SOLUBLE PROTEINS INDUCED BY LOW TEMPERATURE TREATMENT IN THE LEAVES OF SPRING AND WINTER WHEAT CULTIVARS G. KARIMZADEH*, G. R. SHARIFI-SIRCHI, M. JALALI-JAVARAN, H. DEHGHANI AND D. FRANCIS1 Abstract: Quantitative and qualitative changes in total leaf soluble proteins
were determined in a spring cv. Zagross and in a winter cv. Azar 2 cultivar
of allohexaploid wheat (Triticum aestivum L.) exposed to 4°C
for two weeks. The data obtained were used to test whether these different
genotypes of wheat responded differentially to cold treatment. Seedlings
were grown in a controlled growth chamber for 14 d at 20°C and then
transferred to 4°C for 14 d before returning them to 20°C (cold
treatment), or else they were maintained continuously at 20°C (control
treatment). The plants were sampled every 48 h for total leaf fresh
weight measurements. Total leaf soluble proteins were extracted. Proteins
concentration was either determined by a colorimetric method, or size-fractionated
on SDS-PAGE. Clear cold-induced increases in proteins quantity occurred
during the low temperature treatment irrespective of cultivar. However,
the electrophoretic protein patterns showed differences between-cultivar
and between-temperature treatment. Ten new cold-induced polypeptides
(17, 19, 30, 77, 83, 90, 100, 166, 180 and 200 kDa) were produced from
2 d at 4°C reaching their maximum amounts on 6-10 d at 4°C regardless
of cultivar. With increasing exposure to 4°C, fewer new cold-induced
higher molecular weight (HMW) polypeptides (166, 180 and 200 kDa) was
observed compared with those produced over the first week regardless
of cultivar. During the second week, many new cold-induced lower molecular
weight (LMW) polypeptides were detected at 4°C. This alteration
in polypeptide composition from HMW to LMW occurred about 4 d earlier
in the cold-treated seedlings of winter wheat compared with spring wheat.
Perhaps cold-shock proteins are a component of this cold-induced response.
Plant Breeding Department, College of Agriculture, Tarbiat Modarres
University,
PO. Box 14115-336; Fax: +9821 44196524, Tehran, Iran. |
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