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CHLOROPLAST rps8 GENE OF COTTON REVEALS THE CONSERVED NATURE THROUGH OUT PLANT TAXA

TAYYABA SHAHEEN, MEHBOOB-UR-RAHMAN AND YUSUF ZAFAR

Abstract: Chloroplast genome sequences have been utilized extensively for studying evolutionary dynamics, indicating a very low rate of base substitutions among different plant taxa. An initiative has been taken to reveal phylogenetic relationship using rps8 gene derived from chloroplast genome of Gossypium arboreum L. (A genome species) and also to validate the authenticity of A genome as a mother parent in the present day cotton species Gossypium hirsutum L. The rps8 gene sequence derived from G. arboreum was aligned with the DNA sequences of the corresponding rps8 genes of 34 diverse angiosperms. Differences in rps8 gene of G. arboreum with the corresponding gene sequences were in the range of 0% for G. hirsutum to 17.41% for Epiphagus virginiana. The average nucleotide differences among all the rps8 genes were 12.5%. Cluster analysis grouped all the members into three major clusters and one small cluster. Most of the plant species belonging to the same family grouped in one cluster. It has been demonstrated that the A genome is the mother parent in the evolution of G. hirsutum, and the gene is relatively conserved across the different plant taxa.

Plant Genomics and Molecular Breeding (PGMB) Lab, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan
Corresponding Author: Mehboob-ur-Rahman; mehboob_pbd@yahoo.com