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IN VITRO PROPAGATION OF GLADIOLUS ANATOLICUS (BOISS.) STAPF

YELDA EMEK* AND BENGI ERDAG

Abstract: An In vitro propagation method was developed for Gladiolus anatolicus (Boiss.) Stapf (Iridaceae ) using corm sections. Calli were obtained from longitudinally shaped corm sections in Murashige & Skoog medium supplemented with different concentrations of NAA and without growth regulators. The highest rate of callus formation (75 %) occured in MS medium containing 8.5 mg l^-1 NAA. Eight weeks after the culture was initiated, calli in 1 cm diameter were taken into regeneration experiments. In these experiments, shoot regeneration was promoted using NAA, BA only, and their combinations. The highest number of shoots per explant was obtained in MS medium containing 0.2 mg l^-1 BA and 2 mg l^-1 NAA (4.7 shoots per explant). Corm formation in the base of shoots were observed in the medium with 0.1 mg l^-1 BA. Additionally, 5-6 cormel per shoot occured during subculturing. Rooting was obtained in the same medium, but rooting rates in shoots were very low (% 20).
 

Department of Biology, Faculty of Science & Arts Adnan Menderes University, 09010 Aydin, Turkey.
* E mail: yelda@adu.edu.tr, berdag@adu.edu.tr