Pak. J. Bot., 39(1): 73-79, 2007. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
DETERMINATION OF GENOTOXIC EFFECT OF BORON ON ALLIUM CEPA ROOT MERISTEMATIC CELLS MUHSIN KONUK, RECEP LIMAN AND I. HAKKI CIGERCI Abstract: The effect of boron on the mitotic index of Allium cepa root
meristematic cells were investigated. By using the growth inhibition
test LD50 value was determined first and then different doses of varied
concentrations of boron were introduced to onion tuber roots. Distilled
water was used as control. Since Allium cepa cell cycle is 24
hours, application process was carried out at 12, 24 and 48 hours. Mitotic
index and mitotic phase frequences were calculated separately for each
dose introduced. The most observed abnormalities were c-metaphase, prometaphase
and disturbed anaphase-telophases. In addition to these, anaphase bridge,
poliploidy and late chromosome particules were also observed. In the
interphase disturbed nuclei, micronucleus and binuclear cells were determined.
The data were analysed by use of chi-square and student t-test. According
to the results obtained the genotoxic effect of boron on the test organism
was observed. Biology Department, Faculty of Science and Literature, Afyon Kocatepe University, 03200-Afyonkarshisar, Turkey. |
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