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A
HIGH THROUGHPUT, NON-ORGANIC METHOD FOR PLANT GENOMIC DNA ISOLATION
ATA-UR-REHMAN*,
BEN STODART AND HARSH RAMAN
Abstract:
A
simple and reliable method for extracting genomic DNA from leaf and seed
of monocotyledonous and dicotyledonous plants and ascomycete fungal
mycelium is described. The method requires only 50mg of sample and
yields approximately 10µg of high quality DNA. It involves inexpensive,
non-organic constituents and can reliably be used for the parallel
isolation of 384 DNA samples suitable for PCR based marker analysis. The
yield and high quality of extracted DNA from different species, combined
with the use of inexpensive, non-hazardous reagents, provides a
cost-effective method compatible with a 96 well format. The resultant
DNA is suitable for PCR and subsequent fragment analysis using capillary
electrophoresis.
NSW
Department of Primary Industries,
Wagga
Wagga Agricultural Institute, PMB, Wagga Wagga, NSW 2650, Australia.
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