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IN VITRO  REGENERATION  OF  BRASSICA  NAPUS L., CULTIVARS (STAR, CYCLONE AND WESTAR) FROM HYPOCOTYLS AND COTYLEDONARY LEAVES

 HAZRAT ALI¹, ZAHIR ALI², HAIDAR ALI³, SULTAN MEHMOOD² AND WIQAR ALI²

 Abstract: Regeneration protocols were established for Brassica napus L., cultivars viz., Star, Cyclon and Westar. The conditions were optimized for callus induction, shoot and root regeneration. Seeds were surface sterilized using 70% alcohol, 0.01% HgCl₂ and few drops of Tween20 for ten minutes. Which reduced seed borne contamination. Callus induction ability was evaluated by using different concentration of 2, 4-D in combination with 0.5mg/L BAP and 0.5mg/L Silver nitrate, which was used for the first time in MS medium and efficient callus was produced using 0.5mg/L 2, 4-D. Silver nitrate in callus induction media resulted in green callus. Shoots were regenerated on callus using different concentrations of NAA with 2mg/L BAP and 5mg/L Silver nitrate in MS medium. 67-82% shoots were regenerated on media having 0.1mg/L NAA. The shoots were then rooted and best results were obtained on media having 0.3mg/L IBA in half strength MS medium.

¹Department of Biotechnology, Quaid-e-Azam University, Islamabad, Pakistan

²Department of Biotechnology, University of Malakand, NWFP Pakistan

³Department of Botany, University of Karachi, Karachi-75270, Pakistan.