| Pak. J. Bot., 39(4): 1263-1268, 2007. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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CALLUS INDUCTION, PLANT REGENERATION ANDACCLIMATIZATION OF AFRICAN VIOLET (SAINTPAULIA IONANTHA) USING LEAVES AS EXPLANTS SAIFULLAH KHAN*, SAIMA NASEEB AND KASHIF ALI Abstract: African violet (Saintpaulia ionantha) is a very famous ornamental plant due to its various colors and shapes. The present study was conducted to investigate the callus induction and plant regeneration efficiency of African violet and subsequent acclimatization of the regenerated plants. Callus induction efficiency was high with 1.0 mg/L of NAA with MS medium and cotton as a supporting material. Shoot regeneration and multiplication was maximum on full strength MS medium with 3.0 mg/L of BAP and 1.0 mg/L of NAA. Simple MS medium was found effective for the induction of roots in the in vitro grown plantlets. Among the different potting mixes used for the acclimatization of rooted plantlets, 100 % sand was found the best. Maximum survival rate was achieved when plantlets were transplanted during winter. Plant Tissue Culture & Biotechnology Division, H.E.J. Research Institute of Chemistry University of Karachi, Karachi-75270, Pakistan. |
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