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DETECTION OF RIBOSOMAL DNA SITES IN CHICKPEA (CICER ARIETINUM L.) AND MUNGBEAN (VIGNA RADIATA (L.) WILTZEK) BY FLUORESCENCE IN SITU HYBRIDIZATION

G.S.S. KHATTAK¹, E. WOLNY² AND I. SAEED¹

Abstract: Fluorescence in situ hybridization (FISH) technique was used to detect rDNA sites in chickpea and mungbean. In chickpea, the rDNA sites were detected on three pairs of chromosomes. Among these three pairs of chromosomes, one pair exhibited both 25S rDNA and 5S rDNA sites, while in case of other two pairs of chromosomes the 25S rDNA and 5S rDNA sites were located separately on separate pair of chromosomes. In case of mungbean, rDNA sites were found on four pairs of chromosomes. Each 25S rDNA and 5S rDNA had separate sites on two pairs of chromosomes. Active Nucleolus Organizer Regions (NORs) of both the crops were detected through silver staining technique. One and two pairs of chromosomes were detected active for NORs in chickpea and in mungbean, respectively.
 

¹Nuclear Institute for Food and Agriculture (NIFA), P. O. Box 446, Tarnab, Peshawar, NWFP, Pakistan, Email: gssktt@yahoo.com
²Department of plant anatomy and cytology, University of Silesia, Jabiellonska 28, 40-032 Katowice, Poland