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GENETIC DIVERSITY AND GEOGRAPHIC RELATIONSHIP AMONG LOCAL AND EXOTIC CHICKPEA GERMPLASM

MOHAMMAD NISAR¹, A. GHAFOOR², M. RASHID KHAN³, HABIB AHMAD^4*, AFSARI SHARIF QURESHI³ AND HAIDAR ALI⁵

Abstract: The chickpea (Cicer arietinum L.) germplasm, comprising of 118 accessions were evaluated for total seed protein, using SDS-PAGE. Both local and exotic germplasm were used for elaborating genetic diversity and geographic relationships of the accessions. Seed protein profile revealed that variations in major bands were there in accessions 52352, 52530, 52607, 52672, 52670 and 53484. Where as variation in minor bands were visible in most of the accessions. Cluster analysis revealed that the recorded genetic diversity was actually due to the analyses of different gene pools, which might be due to varying degree of out breeding. Geographic relationship based on total seed protein profiles provided clues of introduction of the same germplasm in different areas and transgression of genes into different landraces.

¹Department of Genetics, Hazara University Mansehra, Pakistan

²Institute of Agri-Biotechnology and Genetic Resources, National Agricultural Research Center, Islamabad

³Department of Biological Sciences, Quaid-i-Azam University Islamabad, Pakistan

^*4Department of Botany, Hazara University Mansehra, Pakistan

⁵Department of Botany, University of Karachi, Karachi, Pakistan