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FFECT OF PERMANENT AND TEMPORARY IMMERSION
SYSTEMS ON BANANA MICRO-PROPAGATION
IKRAM-UL-HAQ* AND MUHAMMAD UMAR DAHOT
Abstract: For the establishment of a
micro-propagation protocol for banana (Musa spp.) cv
Basrai, meristematic stem cuttings were used as an explant. A number
of cultures were maintained on MS medium supplemented with various
auxins and cytokinins, of which a combination of IAA and BA for
organogenesis and BA only for shoot induction/multiplication were
considered as good agents for In-vitro propagation of banana.
Micro-propagation efficiency was significantly (P >0.005)
increased, when organogenesis was carried out by culturing on MS medium
supplemented with 10.0
mM
BA; 15.0 mM
IAA and solidified with 3.60 g/L phytagel for
3-weeks, while shoot induction (1.0 g/L phytagel) and its multiplication
(2.0 g/L phytagel) on MS medium supplemented with 10.0
mM
BA for 2 and 3-weeks respectively. 17.65±0.50 plantlets per micro-stem
cutting were developed through this protocol. Among others, in one
medium (6.0 mM
TDZ and 4.0 mM
NAA or/and 10.0
mM
BA) callus formation was observed but later on cultures proceeded to
death, instead of multiplication. The phenolic oxidation was inhibited
through the addition of L-cystein (30.0 mg/L) in each culture. Roots
developed within 2-weeks, by culturing on MS basal medium supplemented with IBA (0.1 mg/L).
Through this protocol, complete and normal micro-propagated plantlets
were obtained within 2-3 months.
Institute of Biotechnology and Genetic
Engineering (IBGE), University of Sindh, Jamshoro, Pakistan.
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