Pak. J. Bot., 39(7): 2717-2724, 2007.

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			Updated: 09-07-09
	BIOSYNTHESIS OF ACID PROTEASES BY PENICILLIUM GRISEOROSEUM IH-02 IN SOLID-STATE FERMENTATION IKRAM-UL-HAQ AND HAMID MUKHTAR Abstract: The present investigation describes the production of acid proteases by Penicillium griseoroseum IH-02 using solid-state fermentation technique. The enzyme biosynthesis was maximum (8.2 U/ml) when fermentation was carried out on a substrate containing soybean meal and wheat bran mixed at a ratio of 3:1 and moistened with D₄ diluent. The substrate to diluent ratio was found to be optimum at 1:2. The extraction of the enzyme from fermented broth was carried out with different extractant and distilled water having pH 5.0 was found to be the best extractant of the enzyme. Institute of Industrial Biotechnology, G.C. University, Lahore, 54000, Pakistan.

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