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EFFECT OF GROWTH HORMONES ON MICROPROPAGATION OF VITIS VINIFERA L. CV. PERLETTE

MUHAMMAD J. JASKANI¹, HAIDER ABBAS², R. SULTANA¹, M.M. KHAN¹, M. QASIM¹ AND IQRAR A. KHAN¹

Abstract: In vitro propagation of Vitis offers opportunities for increasing plant material for cultivation. Cultures were established and maintained in vitro on MS medium supplemented with BA (0, 5 and 10 µM) for shoot and NAA (0, 1, 5 and 10 µM) for callus induction. The best explant sterilization was achieved with 10% chlorox treatment either for 10 or 15 minutes. Oxidative browning was effectively controlled by three subcultures of explants on fresh media. Better shooting was observed on MS medium with 5 µM BA. Shoot proliferation (80%) was obtained by subculturing the micro cuttings on same media as used for shoot formation. Maximum rooting (80%) occurred on medium with 10 µM IBA. The highest callus induction was 80% from stem segments on 5 µM BA followed by leaf disk explants on NAA (1 µM). All callus cultures initiated aerial roots. Leaf disk explants (60%) developed embryoids on MS medium supplemented with NAA (2 mg/l).

¹Institute of Horticultural Sciences, University of Agriculture Faisalabad-38040, Pakistan,

²Dr. A. Q. Khan Institute of Biotechnology and Genetic Engineering, University of Karachi, Karachi-75270, Pakistan